Journal: Cell

Article Title: Protection from SARS-CoV-2 Delta one year after mRNA-1273 vaccination in rhesus macaques coincides with anamnestic antibody response in the lung

doi: 10.1016/j.cell.2021.12.002

Figure Lengend Snippet: mRNA-1273 provides durable protection in the lower airway from B.1.617.2 (A and B) Representative images of lung samples 7 days after B.1.617.2 challenge from 4 NHPs that received mRNA-1273 (A) or mRNA control (B). Top row, detection of SARS-CoV-2 antigen by immunohistochemistry with a polyclonal anti-N antibody. Antigen-positive foci are marked by red arrows. Bottom row, hematoxylin and eosin (H&E) staining illustrating the extent of inflammation and cellular infiltrates. Images at 10× magnification with black bars for scale (100 μm). (C) SARS-CoV-2 antigen and inflammation scores in the left cranial (Lc) lobe, right middle (Rmid) lobe, and right caudal (Rc) lobe of the lungs 7 days after B.1.617.2 challenge. Antigen scoring legend: –, no antigen detected; +/−, rare to occasional foci; +, occasional to multiple foci; ++, multiple to numerous foci; +++, numerous foci. Inflammation scoring legend: –, minimal to absent inflammation; +/−, minimal to mild inflammation; +, mild to moderate inflammation; ++, moderate to severe inflammation; +++, severe inflammation. Horizontal rows correspond to individual NHPs depicted above (A and B).

Article Snippet: Goat anti-human IgD-FITC (polyclonal) , Southern Biotech , Cat#2030-02; RRID: AB_2795624.

Techniques: Control, Immunohistochemistry, Staining

Journal: Cell

Article Title: Protection from SARS-CoV-2 Delta one year after mRNA-1273 vaccination in rhesus macaques coincides with anamnestic antibody response in the lung

doi: 10.1016/j.cell.2021.12.002

Figure Lengend Snippet:

Article Snippet: Goat anti-human IgD-FITC (polyclonal) , Southern Biotech , Cat#2030-02; RRID: AB_2795624.

Techniques: Labeling, Virus, Neutralization, Recombinant, Transfection, Staining, Multiplex Assay, Diagnostic Assay, Software

Journal: Clinical immunology (Orlando, Fla.)

Article Title: Phenotypes and distribution of mucosal memory B-cell populations in the SIV/SHIV Rhesus macaque model

doi: 10.1016/j.clim.2014.04.017

Figure Lengend Snippet: Antibodies used for Flow Cytometry

Article Snippet: Analysis was performed in FlowJo, and data were exported into Excel and Graphpad Prism 6. table ft1 table-wrap mode="anchored" t5 caption a7 Antigen Color Clone Host Species Isotype Supplier CD2 Qdot605 S5.5 Mouse IgG 2 a Invitrogen CD3 BV605 SP34-2 Mouse IgG 1 ,λ BD Bioscience CD14 Qdot605/Qdot800 Tu14 Mouse IgG 2 a Invitrogen CD19 PeCy5 J3-119 Mouse IgG 1 Beckman Coulter CD20 eF650NC 2H7 Mouse IgG 2 b,κ eBioscience CD21 PeCy7 B-ly4 Mouse IgG 1 ,κ BD Bioscience CD27 PerCP-eF710 O323 Mouse IgG 1 ,κ eBioscience CD39 BV421 A1 Mouse IgG 1 ,κ Biolegend CD138 Pe/APC DL-101 Mouse IgG 1 ,κ Biolegend CD184 (CXCR4) BV421 12G5 Mouse IgG 2 a,κ Biolegend CD196 (CCR6) Pe 11A9 Mouse IgG 1 ,κ BD Bioscience CDw199 (CCR9) FITC 112509 Mouse IgG 2 a R&D Systems α4β7 APC A4B7 Rhesus recombinant IgG 1 NHP Reagent Resource IgA FITC Polyclonal Fab Goat NA Southern Biotech IgD Texas Red * Polyclonal Goat NA Southern Biotech IgG APC-Cy7 G18-145 Mouse IgG 1 ,κ BD Bioscience IgM APC G20-127 Mouse IgG 1 ,κ BD Bioscience Ki-67 Ax700 B56 Mouse IgG 1 ,κ BD Bioscience IRF-4 eFluor660/FITC 3E4 Rat IgG 1 ,κ eBioscience HLA-DR Qdot800 Tu36 Mouse IgG 2 b Introgen Viability Dye Aqua NA NA NA Invitrogen Open in a separate window * Green laser (532mm) or yellow-green laser (561nm) required Antibodies used for Flow Cytometry

Techniques: Recombinant

Journal: Clinical immunology (Orlando, Fla.)

Article Title: Phenotypes and distribution of mucosal memory B-cell populations in the SIV/SHIV Rhesus macaque model

doi: 10.1016/j.clim.2014.04.017

Figure Lengend Snippet: Expression of CXCR4 on naïve (panel A) and tissue-like memory (panel B) B-cell populations was compared between tissues. No significant differences were observed among naïve cells of the three tissues. Significantly higher CXCR4 expression frequencies were seen in tissue-like memory B-cells of rectum vs duodenum (p=0.0061). Within each tissue CXCR4 was significantly more highly expressed on naïve B-cells compared to tissue-like memory cells (p<0.0001 for all; panel C). Expression of CCR6 on naïve (panel D) and tissue-like (panel E) memory B-cell populations was compared between tissues. The only significant difference was observed between jejunum and rectum in the naive B-cell population (p = 0.0088; panel D). Within each tissue CCR6 was significantly more highly expressed on naïve B-cells compared to tissue-like memory cells (p<0.0001 for all; panel F). Expression of CCR9 on naïve (panel G) and tissue-like memory (panel H) B-cell populations was compared between tissues. No significant differences were observed between tissues. Within each tissue CCR9 was significantly more highly expressed on tissue-like memory B-cells compared to naive cells (p<0.0001 for all; panel I). Expression of α4β7 on naïve (panel J) and tissue-like (panel K) memory B-cell populations was compared between tissues. No significant differences were observed between tissues. Within each tissue α4β7 was significantly more highly expressed on tissue-like memory B-cells compared to naive cells of duodenum and jejunum (p<0.0023 for both) and rectum (p<0.0001; panel L). (M) CXCR4, (N) CCR6, (O) CCR9 and (P) α4β7 expression on B-cell memory populations of PBMC. * indicates significant differences. Error bar = SEM.

Article Snippet: Analysis was performed in FlowJo, and data were exported into Excel and Graphpad Prism 6. table ft1 table-wrap mode="anchored" t5 caption a7 Antigen Color Clone Host Species Isotype Supplier CD2 Qdot605 S5.5 Mouse IgG 2 a Invitrogen CD3 BV605 SP34-2 Mouse IgG 1 ,λ BD Bioscience CD14 Qdot605/Qdot800 Tu14 Mouse IgG 2 a Invitrogen CD19 PeCy5 J3-119 Mouse IgG 1 Beckman Coulter CD20 eF650NC 2H7 Mouse IgG 2 b,κ eBioscience CD21 PeCy7 B-ly4 Mouse IgG 1 ,κ BD Bioscience CD27 PerCP-eF710 O323 Mouse IgG 1 ,κ eBioscience CD39 BV421 A1 Mouse IgG 1 ,κ Biolegend CD138 Pe/APC DL-101 Mouse IgG 1 ,κ Biolegend CD184 (CXCR4) BV421 12G5 Mouse IgG 2 a,κ Biolegend CD196 (CCR6) Pe 11A9 Mouse IgG 1 ,κ BD Bioscience CDw199 (CCR9) FITC 112509 Mouse IgG 2 a R&D Systems α4β7 APC A4B7 Rhesus recombinant IgG 1 NHP Reagent Resource IgA FITC Polyclonal Fab Goat NA Southern Biotech IgD Texas Red * Polyclonal Goat NA Southern Biotech IgG APC-Cy7 G18-145 Mouse IgG 1 ,κ BD Bioscience IgM APC G20-127 Mouse IgG 1 ,κ BD Bioscience Ki-67 Ax700 B56 Mouse IgG 1 ,κ BD Bioscience IRF-4 eFluor660/FITC 3E4 Rat IgG 1 ,κ eBioscience HLA-DR Qdot800 Tu36 Mouse IgG 2 b Introgen Viability Dye Aqua NA NA NA Invitrogen Open in a separate window * Green laser (532mm) or yellow-green laser (561nm) required Antibodies used for Flow Cytometry

Techniques: Expressing